Fig. 3

HR deficient ovarian cancer cell lines derived from patients show higher sensitivity to IGF-1Rki. Over activation of IGF-1R pathway was observed by western blotting in cells having no BRCA1 expression (a). HR functionality was determined using RAD51 foci (green) formation following cisplatin treatment. A representative image (magnification 100X) is shown in (b), and quantification in (c) (cells with >5 RAD51 foci/nucleus were considered positive). d LC50 of the IGF-1Rki was determined in these cells using the Alamar survival assay e) Correlation was assessed between the LC50 of IGF-1Rki and the HR functionality of cells. The HR functionality was determined by the difference between the % of cells forming RAD51 foci after cisplatin treatment and the control. All the results represent the average of three independent experiments. *p value <0.05