Fig. 1From: Association of activated Gαq to the tumor suppressor Fhit is enhanced by phospholipase CβPLCβs enhance the interaction between Fhit and Gαq. HEK293 cells were co-transfected with different combinations of Flag-Fhit, Gα16, Gα16QL, pcDNA3 vector, PLCβ1, PLCβ2 or PLCβ3. One day after transfection, cell lysates were prepared and immunoprecipitated with anti-Flag affinity gel. PLCβ1, 2, 3, Gα16, Fhit and α-tubulin of the co-immunoprecipitation (IP) assay and total cell lysate (TCL) were determined by Western blottingBack to article page