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Fig. 1 | BMC Cancer

Fig. 1

From: Association of activated Gαq to the tumor suppressor Fhit is enhanced by phospholipase Cβ

Fig. 1

PLCβs enhance the interaction between Fhit and Gαq. HEK293 cells were co-transfected with different combinations of Flag-Fhit, Gα16, Gα16QL, pcDNA3 vector, PLCβ1, PLCβ2 or PLCβ3. One day after transfection, cell lysates were prepared and immunoprecipitated with anti-Flag affinity gel. PLCβ1, 2, 3, Gα16, Fhit and α-tubulin of the co-immunoprecipitation (IP) assay and total cell lysate (TCL) were determined by Western blotting

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