Fig. 4
Piwil1 increased tumor growth potential and enhanced tumor metastatic potential. a The effects of Piwil1 on the cell growth of transfected cells were determined by MTT assay (*P < 0.05, **P < 0.01, ***P < 0.001). b Plated colony forming assays were performed to measure cell proliferation of transfected cells. Representative images are shown. The colonies were counted and graphed (*P < 0.05). c Representative images of spheroid colonies were shown. Scale bars, 100 μm. Quantification of the absolute number of spheroids formed by IshikawashPiwil1 cells or HEC-1BexPiwil1 cells compared to the control cells after 5 days (*P < 0.05). d Cell migration and invasion was measured in transwell chambers. Quantification of migration after 24 h and quantification of invasion after 48 h. Data are reported as fold induction vs control cells. Scale bar, 100 μm. **P < 0.01 Representative images are shown