Fig. 3

Piwil1 led to increased acquisition of endometrial cancer stem cell markers. a RT-qPCR and western blot demonstrated expression level of CD44, ALDH1, CD133, Oct4 and Nanog in Ishikawa^NT and Ishikawa^shPiwil1 cells (*P < 0.05). b RT-qPCR and western blot demonstrated expression level of CD44, ALDH1, CD133, Oct4 and Nanog in HEC-1B^EV and HEC-1B^exPiwil1 cells (*P < 0.05, **P < 0.01). c Representative immunofluorescence images showing CD44, ALDH1 and CD133 expression in Ishikawa^NT and Ishikawa^shPiwil1 cells or in HEC-1B^EV and HEC-1B^exPiwil1 cells. Nuclei were stained with DAPI. Scale bars, 25 μm