Table 1 List for primer pairs used in qPCR
From: Molecular mechanism of anti-cancer activity of phycocyanin in triple-negative breast cancer cells
Gene | Orientation | Primer sequence 5′ TO 3′ | Accession number |
|---|---|---|---|
CYCLIN E | F | TCAGGGTATCAGTGGTGCGA | XM_005259370.2 |
R | CAAATCCAAGCTGTCTCTGTG | ||
CDK-2 | F | TTTGCTGAGATGGTGACTCG | NM_001798.4 |
R | CTTCATCCAGGGGAGGTACA | ||
P21 | F | AAG ACC ATG TGG ACC TGT | NM_001291549.1 |
R | GGT AGA AAT CTG TCA TGC TG | ||
BAX | F | TGCTTCAGGGTTTCATCCAG | NM_001291428.1 |
R | GGCGGCAATCATCCTCTG | ||
Bcl-2 | F | AGGAAGTGAACATTTCGGTGAC | NM_000633.2 |
R | GCTCAGTTCCAGGACCAGGC | ||
CASPASE 9 | F | CCAGAGATTCGCAAACCAGAGG | XM_011542273.1 |
R | GAGCACCGACATCACCAAATCC | ||
MCL1 | F | GGGCAGGATTGTGACTCTCATT | NM_001197320.1 |
R | GATGCAGCTTTCTTGGTTTATGG | ||
COX-2 | F | GATACTCAGGCAGAGATGATCTACCC | NM_000963.3 |
R | AGACCAGGCACCAGACCAAAGA | ||
VEGFR2 | F | TGCCTACCTCACCTGTTTC | NM_002253.2 |
R | GGCTCTTTCGCTTACTGTTC | ||
MMP-9 | F | ACCTCGAACTTTGACAGCGAC | NM_004994.2 |
R | GAGGAATGATCTAAGCCCAGC | ||
GAPDH | F | ACCCAGAAGACTGTGGATGG | NM_001289746.1 |
R | CAGTGAGCTTCCCGTTCAG |