Fig. 2
Oxalate induces breast cancer cell proliferation. Proliferation was performed using a colorimetric assay and following the manufacturing instructions (CyQUANT, Life Technologies) in cells (a) after 3 days of treatment of MCF-7, MDA-MB231, HEK-293 or 7 days of treatment of MCF-10A cells (a) as indicated with oxalate or with acetic acid, or (b) after 3 weeks of treatment in of MCF-7 cells. Cells were cultured in DMEM medium plus an additional specific reagent or not, according to each condition, as indicated. Con: Control, no additional reagent was added; FBS: fetal bovine serum, Ox: oxalic acid or A.A.: acetic acid were added to the culture medium. Results are expressed as DNA content (arbitrary units) found after seeding 4 x103 cells/well.. Bars represent the standard error of the mean of four independent experiments performed in triplicate. A.U.: arbitrary units. Statistical significance determined by Two-way ANOVA with Holm-Sidak’s multiple comparison test (α = 0.05) were performed in experiments graphed in Fig. 2a. One- way ANOVA with Holm-Sidak’s test was performed in Fig. 2b. ****:P value <0.001; **P value <0.01; *P value < 0.05