Fig. 4
From: Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells
LMW Tropomyosin expression and localization are unaltered by hypoxia. Normoxic (N) and hypoxic (H) SH-EP lysates were separated by SDS-PAGE and immunoblotted with a WD4/9d, b γ9d and c CG3 antibodies to examine expression of LMW isoforms Tm4, Tm5NM1/2 and Tm5NM1-11, respectively. Actin used as loading control (d-f) Densitometry was normalized to actin, before expressing hypoxic levels relative to normoxic values (dashed line). Hypoxia did not significantly alter the expression of these LMW isoforms. Results are mean ± SEM; n = 4. g-n SH-EP cells grown for 72 h ± hypoxia were fixed, permeabilized and incubated with γ9d to detect Tm5NM1/2 (g-j) and WD4/9d to detect Tm4 (k-n). Cells then incubated with Alexa555-tagged goat anti-mouse or Alexa488-tagged goat anti-rabbit, respectively. Hypoxia did not alter the intracellular organization of Tm5NM1/2 (h vs. j) nor Tm4 (l vs. n). Independent experiments (γ9d, n = 2; WD4/9d, n = 3). Scale bar = 50 μm, 10 μm in inset