Fig. 1
From: Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells
Hypoxia alters the behavior of SH-EP neuroblastoma cells. a SH-EP cells were grown in normoxic (20 % O2) and hypoxic conditions (1 % O2) for 24–144 h. Hypoxic cell viability normalized to normoxic controls (dashed line through 1). b-i Representative images of a chamber slide (8-well) coated with an extracellular matrix mimic, GFP-tagged gelatin, seeded with 1.6 × 104 SH-EP/well. After 72–96 h ± hypoxia, cells fixed in methanol and stained with DAPI and TRITC-phalloidin. Five fields of view (20× objective) were obtained for each well using a widefield microscope and images imported into Image J for analysis. DAPI nuclear counts (b, c) provided cell numbers. Thresholding on phalloidin (d, e) and the absence of GFP (f, g) provided cell and matrix degradation areas, respectively. Hypoxia resulted in decreased gelatin degradation per cell area at 72 h (h), although no significance was observed at 96 h (i). 1,000+ cells analysed per timepoint. Data is mean ± SEM; n = 4 (a), n = 3 (b-i). * P < 0.05, ** P <0.01 (t-test). Scale bar = 50 μm