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Fig. 3 | BMC Cancer

Fig. 3

From: MiR-449a suppresses the epithelial-mesenchymal transition and metastasis of hepatocellular carcinoma by multiple targets

Fig. 3

Silencing endogenous miR-449a promotes cell motility and induces the EMT phenotype. a The invasive properties of the LO2 cells transfected with between anti-miR-NC and anti-miR-449a were analyzed by an invasion assay using a Matrigel Invasion Chamber. Migrated cells were plotted as the average number of cells per field of view from 3 different experiments, as described in Methods. b Anti-miR-449a- LO2-transfected cells showed higher motility in a wound-healing assay. 48 hours posttreatment. c Cell morphyology of anti-miR-NC- LO2 and anti-miR-449a- LO2 cells. d Expressions of epithelial markers α-catenin, β-catenin and mesenchymal markers fibronectin, N-cadherin and vimentin were compared by western blot analysis between anti-miR-NC-LO2 and anti-miR-449a- LO2 cells. α-tubulin was used as a loading control. e IF was used to compare expression level/pattern of epithelial markers and mesenchymal markers between anti-miR-NC-LO2 and anti-miR-449a-LO2 cells. Epithelial markers α-catenin, β-catenin (red signal) were downregulated in anti-miR-449a cells; mesenchymal markers fibronectin, N-cadherin and vimentin (red cytoplastic signal) were upregulated in anti-miR-449a cells

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