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Fig. 2 | BMC Cancer

Fig. 2

From: Expression of Oct3/4 and Nanog in the head and neck squamous carcinoma cells and its clinical implications for delayed neck metastasis in stage I/II oral tongue squamous cell carcinoma

Fig. 2

Expression of stem cell markers in SP and MP cells. a: The mRNA expressions of Oct3/4, Nanog, Sox2, Notch1, Bmi-1, ABCG2, and GAPDH in both SP and MP cells were separately analyzed using RT-PCR. b: The PCR products visualized under UV-light were semi-quantified using ImageJ. The relative expression levels of the genes were compared after normalization using those of GAPDH. Gene expression levels in the MP cells were defined as 1.0 in each comparison for convenience. c and d: The protein expressions of Oct3/4 and Nanog in both SP and MP cells were separately evaluated using immunofluorescent staining in the SCC4 (c) and SAS (d) cells. Nuclei were stained with DAPI. Scale bar: 100 μm

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