Fig. 5
Antitumor effects of human Ad-cycE on murine ED-1 tumor in FVB mice. Mice carrying established ED-1 tumors were treated with control virus AdGFP or Ad-cycE on days 0, 2, 4, and 6. The total viral dose was 6 x 109 IFU/mouse. (a) The vertical arrow represents each treatment. Tumor volume (V) was plotted against time and was determined by the equation V = (L × W2) / 2, in which L represents the length, and W represents the width of the tumor. The values represent the mean tumor volumes ± S.D. * P < 0.05 compared with AdGFP control group (n = 5), Student’s t-test. (b) H&E staining and immunohistochemical staining of tumor sections using anti-adenoviral hexon antibody. The representative photographs were taken at original magnification X200. Arrows in panel ii point to formed, condensed structures in the tumor tissues. Arrows in panel iv point to strong hexon staining. (c) The magnified views show the progression of pathologic morphology in the sections from mice treated with Ad-cycE