Fig. 4

Characterization of human adenoviral replication on murine cells. (a) 3.5 MOI was used to infect A549 cells, and 10 MOI was used to infect ED-1 cells to achieve the similar infection efficiency. Virus DNA synthesis was determined by Southern blot analyses. Multiple bands in the range of 35–46 kDa were generated from the alternative splicing of E1A transcripts. (b) Cells were mock-infected or infected with AdGFP, Adwt, dl1520, or Ad-cycE at 3.5 MOI (for A549 cells) or 10 MOI (for ED-1). Cells were collected at 24 h p.i., and the cell lysates were immunoblotted for E1A protein, viral capsid protein, and actin. Actin was used as a loading control. (c) The virus yields were determined at 0, 24, 48, and 72 h p.i. with the infection unit method. The values represent the means ± S.D. of triplicate samples