Fig. 2

Infection efficiency of human adenoviruses on A549 and ED-1 cells. (a) A549 cells were cultured with 1 % FBS, and ED-1 cells were cultured with 0 % FBS at a density of 10⁵ (cells/well) and infected with increasing MOI of AdGFP after seeding for 4 h. For each infection, three random fields were taken by EVOS fluorescence microscope (AMG, Bothell, WA) at 72 h post-infection (p.i.). The numbers of GFP cells on each photo were calculated by ImageJ (US National Institutes of Health, Bethesda, MD). The numbers of GFP-positive cells were divided by total cell numbers on each photo to determine the infection efficiency. All values represent the means ± S.D. of triplicate samples. (b) A549 cells were cultured with 1 % FBS, and ED-1 cells were cultured with 0 % FBS at a density of 10⁶ (cells/well) in 60-mm dishes. Cells were infected with AdGFP at 0, 1.25, 2.5, 5, 10, and 20 MOI, respectively, and harvested at 24 h p.i. The DNA samples were digested with PstI, and then totally loaded into the agarose gel for Southern blot analyses with Ad DNA fragments. The amounts of AdGFP entering cells were quantitated by Gel-pro Analyzer 4.0 software (Media Cybernetics, Bethesda, MD) and presented as integrated optical density (I.O.D.) values. (Right, magnified view of boxed section.)