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Table 1 Apoptosis induced by bortezomib, Aplidin and the Aplidin derivatives PM01215 and PM02781; apoptotic cells were determined by Annexin V positive staining and flow cytometric analysis; results are displayed as % of apoptotic cells (mean ± SEM) on three different donors 24 h after incubation with the respective compounds

From: The Aplidin analogs PM01215 and PM02781 inhibit angiogenesis in vitro and in vivo

HUVEC (n = 3) Untreated 11.0 ± 0.6     
concentration [nM]   bortezomib Aplidin™ PM01215 PM 02781
 10   45.0 ± 14.0* 10.7 ± 1.3 10.3 ± 0.9 11.0 ± 0.6
 50   68.0 ± 11.2* 22.7 ± 2.6* 12.0 ± 0.0 12.7 ± 0.9
 100   73.0 ± 10.8* 34.3 ± 2.7* 17.3 ± 0.3* 23.0 ± 2.6*
 200   78.3 ± 11.5* 56.0 ± 4.5* 31.0 ± 4.0* 44.3 ± 10. 7*
HDFs (n = 3) Untreated 5.3 ± 0.3     
concentration [nM]   bortezomib Aplidin™ PM01215 PM 02781
 10   4.7 ± 0.3 4.3 ± 0.3 4.7 ± 0.7 4.7 ± 0.3
 50   7.7 ± 0.7 5.3 ± 0.7 4.3 ± 0.3 4.7 ± 0.3
 100   10.3 ± 0.7* 9.0 ± 0.4 4.3 ± 0.3 6.3 ± 0.3
 200   12.7 ± 1.7* 12.3 ± 0.7* 6.0 ± 1.0 7.3 ± 1.2
PBMNC (n = 3) Untreated 11.3 ± 0.9     
concentration [nM]   bortezomib Aplidin™ PM01215 PM 02781
 10   9.7 ± 2.3 10.3 ± 1.2 9.7 ± 0.9 10.0 ± 1.5
 50   25.0 ± 1.0* 11.7 ± 1.3 9.7 ± 1.3 10.0 ± 1.2
 100   28.0 ± 2.0* 12.3 ± 1.7 9.7 ± 1.3 11.7 ± 2.3
 200   29.7 ± 2.3* 19.3 ± 1.7* 11.0 ± 1.5 12.7 ± 1.9
  1. Primary cells were human umbilical vein endothelial cells (HUVECs), human diploid fibroblasts from bone marrow (HDFs) and human peripheral blood mononuclear cells (PBMNCs). *Indicates p value < 0.05