Fig. 8

Aplidin analogs inhibit vascularization and growth of human myeloma xenografts in the chicken chorioallantoic membrane (CAM) in vivo. a Human multiple myeloma cell lines (OPM-2) were lentivirally transfected and selected to express stably green fluorescent protein (GFP). Then cells were mixed with primary human bone-marrow mesenchymal cells, collagen type-I as extracellular matrix component and with 1 nmol of Aplidin analogs PM01215 and PM02781. Spheroids (n = 6) were grafted on the chorioallantoic membrane of chicken embryos. Xenografts formed nice vascularized tumors after 4 days; PM02781 and PM01215 treated xenografts were less vascularized. Arrows indicate blood vessel sprouting close to tumor xenograft, bars indicate 500 μm. b Single MM xenografts were excised and homogenized in lysis puffer and thereafter, measured by ELISA. GFP concentrations of single tumors were calculated (n = 6, mean ± SEM; stars indicate p values <0.05, differences between the means of one treatment group and untreated control were analyzed). PM02781 and PM01215 treated myeloma xenografts displayed less tumor cell mass