Fig. 4
From: miR-320b suppresses cell proliferation by targeting c-Myc in human colorectal cancer cells
miR-320b down-regulates c-Myc expression in CRC cells. a Wild-type (WT) and mutant (Mut) of putative miR-320b targeting sequences in c-Myc mRNA. Mutant sequences were shown in bold type. b Analysis of luciferase activity. HEK293 and SW-480 cells were co-transfected with miR-320b mimics (miR-320b) or negative control oligonucleotides (NC), pRL-TK and firefly luciferase reporter plasmid containing putative miR-320b targeting sequences of c-Myc. c, d The endogenous c-Myc mRNA (c) and protein (d) levels were quantified 48 h. after transfection of (miR-320b) and (NC) in HCT-116 and SW-480 cells. e Expression of c-Myc was measured in 48 CRC and adjacent non-tumor tissues by qRT-PCR. f A negative Spearman correlation between miR-320b and c-Myc mRNA levels was found in CRC samples. g Analysis of c-Myc and miR-320b expression in the same CRC and adjacent non-tumor tissue by IHC and qRT-PCR. Brown signal in IHC was considered as positive staining for c-Myc. Scale bar = 50 μm. Data analyzed using Student’s t-test.*p < 0.05, **p < 0.01