Table 3 Sequences of primers and probes of COL11A1 transcript variants
|  | Name | Oligonucleotide Sequence, 5’-3’ |
|---|---|---|
Variant A & C Forward Primer | VARAC F | TGTGAGCATTATAGTCCAGACTGTGA |
Variant E Forward Primer | VARE F | CAGATAGATGAGGCAAACATCG |
Variant B Forward Primer | VARB F | AAGAAGATGAGGACAGTGGCTA |
Variant C Reverse Primer | VARC R | CCATGGCCATTTATCTCCGT |
Variant A, E & B Reverse Primer | VARAEB R | CATATTCGCCTAAATCTCCATCTAC |
Variant A & C Sensor Probe | VARAC FL | TCCTCAGTTACAGTGGGTCCCTCTGTTAC-FL |
Variant A & C Anchor Probe | VARAC LC | LC 640-CTTTCAGCCTCTTTATACTCTGCTTCCCCA |
Variant E & B Sensor Probe | VAREB FL | GCTCATTTGTCCCAGAAATGCC-FL |
Variant E & B Anchor Probe | VAREB LC | LC 640-AGGAGCTTCTGTCTGGTAACTTTCCATTGT |
General COL11A1 Forward Primer | F | AATGGAGCTGATGGACCACA |
General COL11A1 Reverse Primer | R | TCCTTTGGGACCGCCTAC |