Fig. 3

CDKN1A and RAD21 mRNA protectors specifically blocked the silencing effects of miR-17 and miR-92 families on the 3′-UTR of CDKN1A and RAD21. a CDKN1A and RAD21 mRNA protectors that could block the binding of miR-17 and miR-92a to their 3′-UTR were designed respectively. b CDKN1A and RAD21 mRNA protectors rescued the reduction of luciferase activities caused by the miR-17 and miR-92a targeting effect on their 3′-UTR respectively. c The protein and mRNA expression levels of CDKN1A and RAD21 were increased in A549 cells after transfection of CDKN1A and RAD21 mRNA protectors, detected by Western Blotting and RT-PCR assays. d To varying degrees, individual or combined transfection of CDKN1A and RAD21 mRNA protectors led to increased cisplatin resistance of A549 cells significantly. (n = 3, *P < 0.05)