Fig. 3From: Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimensValidation analysis of selected differentially expressed genes. a qRT-PCR of selected genes on B cells (n = 5), CLL specimens, RNA seq cohort (n = 5) and CLL specimens, test cohort (n = 47). Data shown is the delta delta cT relative to actin. (Mean and standard deviation). Table below panel A shows the P-values of the qRT-PCR data for the comparison of B cells and CLL RNA seq cohort (n = 10), and B cells and CLL test cohort. (t-test). b Fold expression of selected genes in the larger CLL cohort (n = 47) based on qRT-PCR analysis. * PTPRK expression was not detected in normal B cells therefore fold change could not be calculatedBack to article page