Fig. 4

GPER, but not ERα, is required for estrogen-and G-1-mediated translocation of FOXO3-GFP. MCF7 cells were transfected with siRNA targeting either GPER (siGPER) or ERα (siERα) or a non-targeting siRNA (siControl), followed by transfection with FOXO3-GFP. Transfected cells were serum starved for 24 h prior to treatments. a Cells were treated with 0.1 % DMSO, 50 nM estrogen or 100 nM G-1 for 15 min. b Cells were treated with 0.1 % DMSO or 50 ng/ml EGF for 15 min. c and d Representative Western blots of cell lysates collected at the same time as the treatments in (a) and (b). Results are reported as mean +/− s.e.m. from at least 3 experiments. *, p < 0.05; **, p < 0.01 vs. DMSO control of the matched siRNA. #, p < 0.05 vs. the ligand-matched siControl treatment