Table 2 Primer sequences, forward and reverse, used for realtime PCR analysis
Gene | Forward primer | Reverse primer |
|---|---|---|
GAPDH | 5′-ACC ACA GTC CAT GCC ATC AC-3′ | 5′-TCC ACC CTG TTG CTG TA-3′ |
SIRT1 | 5′-TGG CAA AGG AGC AGA TTA GTA GG-3′ | 5′-CTG CCA CAA GAA CTA GAG GAT AAG A-3′ |
DNMT1 | 5′-TAC CTG GAC GAC CCT GAC CTC-3′ | 5′-CGT TGG CAT CAA AGA TGG ACA-3′ |
DNMT3A | 5′-TAT TGA TGA GCG CAC AAG AGA GC-3′ | 5′-GGG TGT TCC AGG GTA ACA TTG AGS′ |
DNMT3B | 5′-GGC AAG TTC TCC GAG GTC TCT G-3′ | 5′-TGG TAC ATG GCT TTT CGA TAG GAS′ |
hTERT | 5′-AGG GGC AAG TCC TAC GTC CAG T-3′ | 5′-CAC CAA CAA GAA ATC CAC C-3′ |