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Table 1 Colony forming potential in TNBCs and MCF10A control cells

From: Epigenetic-based combinatorial resveratrol and pterostilbene alters DNA damage response by affecting SIRT1 and DNMT enzyme expression, including SIRT1-dependent γ-H2AX and telomerase regulation in triple-negative breast cancer

Treatments (10 days)

Cells plated

Colony counted

Plating efficiency (%)

Survival fraction (%)

M DA-MB-15 7

    

DMSO

500

490

98

100.00

Res 15

500

399

79.8

81.43

Ptero 5

500

417

83.4

85.10

Combination

500

220

44

44.90

Treatments

Cells plated

Colony

Plating

Survival fraction

(14 days)

 

counted

efficiency

 

MCF10A

    

DMSO

500

165

32.96

100

Res 15

450

136

30.33

92.03

Ptero 5

500

175

35.06

106.3

Combination

480

145

30.13

91.44

  1. After staining the tissue culture dishes (20 mm), colonies were counted using a colony counter. Large colonies consisting of 50 or more cells were counted. MDA-MB-157 breast cancer cells displayed a reduction in colony forming potential after different treatments as determined by survival fraction (%). This reduction was more effective in combination treatments when compared with single doses of resveratrol (15 μM) and pterostilbene (5 μM) alone. Interestingly, MCF10A control cells did not display a significant change in the colony forming potential when compared within the treatment groups and the DMSO treated group. These observations further provide the effectiveness of this combinatorial regimen in inhibiting breast cancer cells growth. However, HCC1806 breast cancer cells did not form any successful colonies in any of the groups (data not shown). Values are representative of three independent experiments