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Fig. 7 | BMC Cancer

Fig. 7

From: Epigenetic-based combinatorial resveratrol and pterostilbene alters DNA damage response by affecting SIRT1 and DNMT enzyme expression, including SIRT1-dependent γ-H2AX and telomerase regulation in triple-negative breast cancer

Fig. 7

Effects of combination and SIRT1 knockdown on γ-H2AX protein expression in HCC1806 breast cancer cell line as analyzed by immunofluorescence microscopy. DAPI was used to stain cell nuclei, shown in blue color. Alexa Fluor®488 (GFP) shown in green was used to stain the target protein. a Shows the expression of γ-H2AX protein in green with live cells stained in blue after 72 h of DMSO treatment in HCC1806 breast cancer cells. b and c The effect of 15 μM resveratrol and 5μM pterostilbene treatments after 72 h are demonstrated. Images show no change in the intensity of blue and green color between the two sets as marked by the red arrows. d Effects of combination after 72 h of treatment depict a significant change in the intensity of green color (γ-H2AX protein) in comparison to the blue color stained nuclei. e Effect of SIRT1 knockdown in HCC1806 breast cancer cells. This resulted in decreased γ-H2AX protein expression (green color) as marked by red arrows

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BMC Cancer

ISSN: 1471-2407

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