Fig. 10

Effects of combination and SIRT1 knockdown, on hTERT expression in HCC1806 breast cancer cells. a Combination (Res/Ptero) treatment after 72 h rendered a significant down-regulation of hTERT mRNA levels as shown by real-time PCR. GAPDH was used as an internal control. Values are representative of three independent experiments ± SE; *P <0.05, **P <0.01. b SIRT1 knockdown (SIRT1 KD) was performed and its effects on hTERT mRNA were analyzed using real-time PCR. After 72 h of knockdown, there was a significant down-regulation of both SIRT1 and hTERT mRNAs. No significant effects were observed with scrambled siRNA. GAPDH was used as an internal control. Values are representative of three independent experiments ± SE; *P <0.05, **P <0.01. c Telomerase enzyme activity was analyzed using TRAP assays in the HCC1806-treated and knockdown set. At the fourth and fifth days of treatment, there was a significant down-regulation of telomerase activity with the combination of resveratrol and pterostilbene. SIRT1 knockdown also resulted in significant down-regulation of telomerase enzyme activity at fifth day. Values are representative of three independent experiments and are shown as percent of control ± SE; *P <0.05, **P <0.01