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Fig. 5 | BMC Cancer

Fig. 5

From: Phosphorylation of a splice variant of collapsin response mediator protein 2 in the nucleus of tumour cells links cyclin dependent kinase-5 to oncogenesis

Fig. 5

Sub-cellular localization of phosphorylated CRMP2. Subcellular fractionation of three cancer cell lines (A549, EBC-1 and H460), and a human neuroblastoma SHSY5Y cell line (positive control for CRMP2 expression) was performed prior to Western blot analysis with the indicated antibodies. GAPDH and histone H4 were used as markers for the successful fractionation of cytoplasm and chromatin-bound nuclear fraction, respectively. Western blots shown are representative of three independent experiments

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