Fig. 4

CDK5 substrate phosphorylation in human tissue. a Nineteen lung tumour biopsies were immunostained with the pCRMP2 S522 antibody and developed with DAB. Tumour sections were designated squamous (11), adenocarcinoma (4) or other (4) and graded using a semi-quantitative scale from 0–3, with 0 representing no staining, 1 representing light brown staining, 2 representing moderate brown staining and 3 representing dark brown staining. Representative images for each grade are provided. Clear nuclear (n) or cytoplasmic (c) staining is indicated, and slides where the staining was specific to tumour tissue rather than healthy tissue are indicted by (t). b A second independent cohort of adenocarcinoma (ADC) and squamous cell carcinoma (SCC) sections (9 of each type) were immunostained with the pCRMP2 Ser522 antibody and scored as in A. Representative histology for each is provided in upper panel (Scale bar = 50 μm), while the individual and average scores for pCRMP2 Ser522 staining is given below as mean ± S.E.M. t-test, *P < 0.05, **P < 0.01, ***P < 0.001