Fig. 4

Areas of tumor infiltrated by immune cells after mEHT and DC treatment. Representative images of immunohistochemical straining revealed that quantities of CD45 (a) and F4/80 (b) were increased in tumors that were treated with DC and mEHT, either alone or in combination. Proportion of positive cells in one field that was randomly selected from ten fields, was calculated. c Amount of eosinophil increased significantly upon treatment of tumor; error bars represent standard errors. (***) P < 0.001 (t-test) relative to control. EH: electro-hyperethermia