Table 3 DNA methylation data of biopsies, macrodissected samples and laser microdissected (LCM) colon epithelial cells

ALDH1A3_assay 1

4.33 ± 2.23

3.59 ± 1.92

5.74 ± 6.26

0.31

0.36

0.21

BCL2_assay 1

3.22 ± 1.12

3.50 ± 2.21

9.74 ± 13.41

0.63

p < 0.05

0.09

BCL2_assay 2

0.91 ± 0.72

0.42 ± 0.41

0.73 ± 0.59

p < 0.05

0.44

0.11

CDX1_assay 1

21.96 ± 9.38

19.41 ± 8.76

16.01 ± 9.83

0.42

0.08

0.32

COL1A2 assay 2

22.67 ± 5.62

37.37 ± 14.36

39.52 ± 18.02

p < 0.01

p < 0.01

0.72

COL1A2_assay 1

8.65 ± 3.63

9.77 ± 6.13

15.31 ± 12.57

0.51

p < 0.05

0.14

CYP27B1_assay 1

10.71 ± 3.14

8.53 ± 4.19

11.37 ± 6.76

0.09

0.71

0.18

CYP27B1_assay 2

4.03 ± 4.16

2.71 ± 1.69

6.45 ± 10.56

0.26

0.36

0.19

CYP27B1_assay 4

45.12 ± 2.66

44.50 ± 2.08

46.70 ± 8.04

0.46

0.42

0.31

ENTPD5_assay 1

2.17 ± 0.62

1.78 ± 0.91

2.93 ± 3.32

0.15

0.35

0.20

FADS1_assay 1

0.75 ± 0.44

0.46 ± 0.23

6.98 ± 10.82

p < 0.05

p < 0.05

p < 0.05

MAL_assay 1

16.46 ± 16.12

38.87 ± 26.52

50.42 ± 26.99

p < 0.01

p < 0.01

0.25

PRIMA1_assay 1

7.25 ± 3.15

9.88 ± 10.60

28.91 ± 22.65

0.30

p < 0.01

p < 0.01

PRIMA1_assay 2

3.99 ± 1.32

6.49 ± 5.60

22.29 ± 20.14

0.06

p < 0.01

p < 0.01

PRIMA1_assay 3

37.96 ± 13.57

48.13 ± 20.69

56.43 ± 21.17

0.11

p < 0.01

0.30

PRIMA1_assay 4

8.30 ± 7.95

17.78 ± 13.26

26.82 ± 25.00

p < 0.05

p < 0.01

0.23

PTGDR assay 1

5.79 ± 1.84

4.09 ± 1.85

11.12 ± 9.36

p < 0.05

p < 0.05

p < 0.01

PTGDR assay 2

11.71 ± 3.51

8.01 ± 4.50

15.58 ± 6.48

p < 0.05

p < 0.05

p < 0.01

PTGDR_assay 3

6.47 ± 9.27

3.61 ± 4.63

10.47 ± 9.51

0.28

0.22

p < 0.05

PTGS2_assay 1

8.82 ± 3.47

12.15 ± 15.01

10.80 ± 13.16

0.34

0.52

0.80

PTGS2_assay 2

4.33 ± 9.01

5.83 ± 10.49

5.64 ± 8.48

0.65

0.67

0.96

SFRP1_assay 1

39.60 ± 18.46

60.82 ± 20.41

54.49 ± 18.47

p < 0.01

p < 0.05

0.38

SFRP2_assay 1

14.84 ± 4.38

38.52 ± 20.06

44.44 ± 20.05

p < 0.01

p < 0.01

0.43

SFRP2_assay 2

20.14 ± 4.48

39.33 ± 17.77

48.04 ± 16.20

p < 0.01

p < 0.01

0.17

SOCS3_assay 1

4.96 ± 1.52

7.57 ± 6.18

12.16 ± 12.01

0.08

p < 0.05

0.20

SOCS3_assay 2

18.24 ± 7.68

49.27 ± 20.49

49.43 ± 18.81

p < 0.01

p < 0.01

0.98

SOCS3_assay 3

8.82 ± 16.20

21.75 ± 21.72

25.79 ± 22.22

0.05

p < 0.05

0.62

SULF1_assay 1

5.32 ± 2.16

6.80 ± 11.30

9.60 ± 8.91

0.57

p < 0.05

0.46

SULF1_assay 2

4.60 ± 4.02

7.98 ± 10.78

11.28 ± 14.82

0.21

0.06

0.49

SULT1A1_assay 1

5.51 ± 4.35

3.25 ± 1.99

7.58 ± 9.46

0.07

0.39

0.09

SULT1A1_assay 2

42.68 ± 5.54

44.86 ± 9.49

48.80 ± 10.07

0.40

p < 0.05

0.28

SULT1A1_assay 3

3.11 ± 2.94

2.14 ± 1.66

6.34 ± 6.55

0.26

0.06

p < 0.05

THBS2_assay 1

18.17 ± 5.03

26.47 ± 13.79

28.36 ± 16.92

p < 0.05

p < 0.05

0.74

THBS2_assay 2

91.32 ± 2.37

85.64 ± 6.49

83.73 ± 6.83

p < 0.01

p < 0.01

0.44

THBS2_assay 3

89.68 ± 1.80

87.51 ± 3.55

83.02 ± 6.69

p < 0.05

p < 0.01

p < 0.05

THBS2_assay 4

22.32 ± 10.74

27.97 ± 13.58

25.47 ± 15.20

0.18

0.48

0.64

TIMP1_assay 1

19.54 ± 14.39

16.65 ± 14.45

13.96 ± 10.07

0.56

0.21

0.56

LCM – colon epithelial cells

ALDH1A3_assay 1

8.30 ± 3.96

4.02 ± 2.56

3.60 ± 3.49

0.08

0.08

0.83

BCL2_assay 1

3.14 ± 1.18

3.30 ± 1.19

4.26 ± 2.58

0.84

0.40

0.47

BCL2_assay 2

4.53 ± 7.59

0.32 ± 0.35

0.33 ± 0.47

0.24

0.30

0.99

CDX1_assay 1

11.26 ± 19.16

1.64 ± 1.16

2.43 ± 2.43

0.29

0.40

0.54

COL1A2 assay 2

19.18 ± 6.95

61.72 ± 18.25

53.46 ± 18.52

p < 0.01

p < 0.01

0.50

COL1A2_assay 1

6.46 ± 3.34

14.06 ± 6.37

22.84 ± 7.00

p < 0.05

p < 0.01

0.07

CYP27B1_assay 1

10.20 ± 12.19

3.28 ± 0.94

7.88 ± 7.06

0.24

0.75

0.19

CYP27B1_assay 2

7.14 ± 8.88

0.50 ± 0.31

6.38 ± 8.64

0.13

0.90

0.17

CYP27B1_assay 4

43.56 ± 2.13

44.90 ± 3.89

45.90 ± 5.26

0.52

0.38

0.74

ENTPD5_assay 1

1.68 ± 0.56

1.74 ± 0.11

1.88 ± 0.60

0.80

0.62

0.62

FADS1_assay 1

12.76 ± 17.16

4.70 ± 7.95

2.94 ± 5.80

0.37

0.26

0.70

MAL_assay 1

11.76 ± 10.80

61.16 ± 40.61

84.68 ± 11.92

p < 0.05

p < 0.01

0.25

PRIMA1_assay 1

4.00 ± 0.85

32.56 ± 31.71

26.42 ± 33.03

0.08

0.17

0.77

PRIMA1_assay 2

2.34 ± 1.13

29.82 ± 22.74

29.45 ± 35.82

p < 0.05

0.13

0.99

PRIMA1_assay 3

21.70 ± 25.92

73.64 ± 28.56

93.55 ± 3.06

p < 0.05

p < 0.01

0.21

PRIMA1_assay 4

0.62 ± 0.73

43.61 ± 29.53

59.91 ± 31.31

p < 0.05

p < 0.01

0.42

PTGDR assay 1

2.95 ± 0.80

3.16 ± 2.52

28.94 ± 10.72

0.87

p < 0.01

p < 0.01

PTGDR assay 2

6.40 ± 3.82

3.56 ± 1.26

23.81 ± 10.40

0.15

p < 0.01

p < 0.01

PTGDR_assay 3

1.63 ± 3.23

1.14 ± 1.45

25.72 ± 20.81

0.77

p < 0.05

p < 0.05

PTGS2_assay 1

5.80 ± 6.58

2.33 ± 0.33

3.05 ± 1.00

0.27

0.44

0.17

PTGS2_assay 2

9.66 ± 8.80

0.83 ± 0.80

18.19 ± 36.43

0.06

0.62

0.32

SFRP1_assay 1

19.03 ± 25.20

78.72 ± 23.36

91.08 ± 5.91

p < 0.01

p < 0.01

0.28

SFRP2_assay 1

12.53 ± 5.01

55.95 ± 31.32

90.78 ± 1.56

p < 0.05

p < 0.01

0.06

SFRP2_assay 2

16.34 ± 9.82

68.90 ± 27.80

85.16 ± 2.56

p < 0.01

p < 0.01

0.29

SOCS3_assay 1

3.86 ± 0.84

6.57 ± 5.39

25.07 ± 14.20

0.30

p < 0.05

p < 0.05

SOCS3_assay 2

14.28 ± 8.30

68.61 ± 35.98

90.36 ± 8.66

p < 0.05

p < 0.01

0.28

SOCS3_assay 3

2.59 ± 2.72

55.24 ± 43.52

85.04 ± 12.02

p < 0.05

p < 0.01

0.23

SULF1_assay 1

4.62 ± 4.06

7.52 ± 8.25

16.12 ± 17.25

0.50

0.18

0.34

SULF1_assay 2

4.75 ± 4.58

8.35 ± 12.00

8.46 ± 4.78

0.59

0.31

0.99

SULT1A1_assay 1

2.54 ± 1.05

4.63 ± 3.81

4.02 ± 2.15

0.27

0.20

0.76

SULT1A1_assay 2

45.35 ± 7.51

38.07 ± 4.36

56.53 ± 26.33

0.10

0.39

0.16

SULT1A1_assay 3

13.90 ± 16.07

7.69 ± 8.11

7.87 ± 12.23

0.47

0.57

0.98

THBS2_assay 1

13.64 ± 6.67

45.55 ± 25.89

49.61 ± 8.64

p < 0.05

p < 0.01

0.75

THBS2_assay 2

90.83 ± 2.02

84.92 ± 8.28

83.33 ± 10.87

0.16

0.17

0.80

THBS2_assay 3

90.16 ± 2.37

87.54 ± 3.22

78.65 ± 8.85

0.18

p < 0.05

0.07

THBS2_assay 4

24.84 ± 18.37

42.88 ± 8.47

42.48 ± 18.50

0.11

0.33

0.97

TIMP1_assay 1

32.62 ± 21.75

15.35 ± 20.90

5.35 ± 2.27

0.24

p < 0.05

0.32