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Fig. 4 | BMC Cancer

Fig. 4

From: Inhibition of IGF1-R overcomes IGFBP7-induced chemotherapy resistance in T-ALL

Fig. 4

Cytostatic-drug treatment of a Jurkat clone overexpressing IGFBP7. Jurkat clones transfected with pCntrl or pIGFBP7 were incubated in the presence of vincristine, etoposide or asparaginase for 24 hours. a: A WST-1 assay was performed and proliferation upon cytostatic drug-treatment determined in relation to the respective untreated control (1). Mean values ± SEM of 4 independent experiments done in triplicates. *P-value ≤ 0.05 (Mann–Whitney-U-test) b: Cells stained with AnnexinV-FITC and 7-AAD were analyzed by flow cytometry. Representative dot plots out of four independent experiments. Percentages of cells within each quadrant are indicated. c: Early apoptotic (AnnexinV+7-AAD) as well as late apoptotic and necrotic cells (AnnexinV+7-AAD+) were assessed as shown (b). Mean values ± SEM/SD of 4 independent experiments done in triplicates. *P-value ≤ 0.05 (Mann–Whitney-U test)

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