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Fig. 3 | BMC Cancer

Fig. 3

From: Inhibition of IGF1-R overcomes IGFBP7-induced chemotherapy resistance in T-ALL

Fig. 3

Cell cycle distribution of Jurkat and Molt-4 clones over-expressing IGFBP7. Clones transfected with pCntrl or pIGFBP7 were cultured for one (Molt-4) or three days (Jurkat). Cell cycle states were analyzed by flow cytometry after BrdU incorporation and subsequent antibody binding in combination with direct 7-AAD staining. a: Representative dot blots of transfected Jurkat cells show the gated populations: BrdU+ cells (upper region) were considered to be in S-phase; BrdU7-AADhigh (lower right region) in G2/M, BrdU7-AADlow in G1/G0 (lower middle region) and BrdU7-AAD apoptotic (lower left region). Percentages of cells within each region are indicated. b: Relative distribution of Jurkat and Molt-4 cells in cell cycle phases. Triplicates of four independent experiments are plotted; lines indicate mean values ± SEM; *P-value ≤ 0.05, **P-value ≤ 0.01, ***P-value ≤ 0.001 (Wilcoxon signed-rank test)

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