Fig. 1

IGFBP7 expression in Jurkat and Molt-4 transfected for over-expression. Jurkat and Molt-4 cells were transfected with pCntrl or pIGFBP7 and cloned for 35 days in the presence of G418. a: IGFBP7 or GAPDH mRNA levels were assessed after additional 24 h culture without G418 by RT-PCR. KG-1a served as control for constitutive IGFBP7 mRNA expression. Images representative for four independent experiments show PCR products separated by agarose gel electrophoresis. b: IGFBP7 mRNA expression was measured by Sybr Green qRT-PCR and normalized to GAPDH as housekeeping control. Mean values ± SEM of five duplicate determinations. **P-value ≤ 0.01 (Mann–Whitney-U test) c: Secreted IGFBP7 protein from Jurkat or Molt-4 clones was quantified from the supernatants by ELISA after four days of culture without G418. Mean values ± SEM of three triplicate determinations