Skip to main content

Advertisement

Table 1 PCR amplification conditions for the MS-HRM assays

From: Assessment of DNA methylation profiling and copy number variation as indications of clonal relationship in ipsilateral and contralateral breast cancers to distinguish recurrent breast cancer from a second primary tumour

Gene MgCl2 concentration (mmolL−1) Primer concentration (nmolL−1) Cycling time (sec) Annealing temperature (°C)
APC 2.5 F - 200 10, 15, 20 58.5
R - 300
BRCA1 4.0 F - 250 10, 10, 20 61.0
R - 250
CDH1 2.5 F - 200 10, 10, 20 63.5
R - 200
CDH13 3.0 F - 300 10, 20, 25 58.0
R - 400
CDKN2A (P16) 2.5 F - 300 10, 10, 20 70.0
R - 200
GSTP1 2.5 F - 200 10, 10, 20 64.5
R - 200
MAL 2.5 F - 200 10, 10, 20 59.0
R - 200
MGMT 4.0 F - 250 10, 20, 20 60.0
R - 250
MLH1 3.5 F - 200 10, 15, 20 59.0
R - 300
TP73 3.0 F - 300 10, 15, 20 55.0
R - 200
RARβ 3.0 F - 200 10, 10, 20 66.0
R - 300
RASSF1A 3.0 F - 300 15, 25, 20 65.0
R - 200
TWIST1 2.5 F - 200 10, 10, 20 52.0
R - 300
WIF1 3.0 F - 400 10, 20, 25 53.0
R - 400