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Fig. 4 | BMC Cancer

Fig. 4

From: PRIMA-1MET induces death in soft-tissue sarcomas cell independent of p53

Fig. 4

Autophagy and JNK phosphorylation induced by PRIMA-1MET. a Western blot and quantification of LC3II in each cell line after 72 hr of PRIMA-1MET. Chloroquine (C) was used at 20 μM, NT: Not Treated, P: PRIMA-1MET, C + P: Chloroquine and Prima-1MET. b IB 134 and IB 138 cells were treated and fixed for immunostaining with an antibody against LC3. Representative images from two independent experiments are shown. c JNK pathway activation assessed by western blot after 96 hr of PRIMA-1MET exposure. For all these assays PRIMA-1MET doses correspond to previously found doses responsive for loss of MMP in 50 % of treated cells assessed by TMRM assays; 30 μM for IB130 and IB134, 25 μM for IB138 and IB136 and 50 μM for IB139

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