Fig. 3
From: PRIMA-1MET induces death in soft-tissue sarcomas cell independent of p53
PRIMA-1MET induces ROS-associated toxicity and loss of MMP. a ROS production estimated by DCF staining and assessed by flow cytometry, not treated in red, PRIMA-1MET 30 μM (IB130) and 10 μM (IB136) for 96 h in blue. b ROS implication in PRIMA-1MET growth suppression effects using MTT assay. Cells were treated for 96 h with range of PRIMA -1MET alone (black) and combined with NAC (2,5 mM) (red). c Loss of MMP by cell line with TMRM fluorescent assay, n = 3, error bars show SEM