Fig. 2
From: PRIMA-1MET induces death in soft-tissue sarcomas cell independent of p53
Cell death induced by PRIMA-1MET on STS cell lines. a caspases 3 and 7 activation in cell lines, upper line represent fusion of FLICA fluorescence in cells non treated (NT) not marked (blue), NT marked (red) and treated by Staurospaurin (orange) at 1 μM (IB134), 5 μM (IB136 and IB139) for up to 72 hr. Lower line represent fusion of FLICA fluorescence in cells NT not marked (blue), NT marked (red) and treated by Prima-1MET (orange) at 60 μM (IB134) or 80 μM (IB136 and IB139) for up to 72 hr. b Levels of the indicated proteins evaluated by Western blot analysis after exposure for up to 72 hours to specific defined cell line IC50 PRIMA-1MET doses. c Cell death analyses using FITC annexin-V and propidium iodide assay. Upper line compare NT condition (red) to PRIMA-1MET at IC50 cell line specific dose (blue) defined by MTT assay, lower line compare NT (red) to 80 μM of PRIMA-1MET except for IB117 (50 μM) (blue)