Table 1 Primers used for RTqPCR. Primer sequences for DNMT1 amplified a region of 88Â bp. Primers for DNMT3a amplified a region of 68Â bp; Primers for DNMT3b amplified a region of 68Â bp; Primers for MYC amplified a region of 129Â bp; Primers for HPRT amplified a region of 191Â bp
From: Burkitt lymphoma beyond MYC translocation: N-MYC and DNA methyltransferases dysregulation
Gene | Primer sequence |
|---|---|
DNMT1-FORWARD | 5’-CGACTACATCAAAGGCAGCAACCTG-3’ |
DNMT1-REVERSE | 5’-TGGAGTGGACTTGTGGGTGTTCTC-3’ |
DNMT3A-FORWARD | 5’-TAT TGATGAGCGCACAAGAGAGC-3’ |
DNMT3A-REVERSE | 5’-GGGTGTTCCAGGGTAACATTGAG-3’ |
DNMT3b-FORWARD | 5’-GGCAAGTTCTCCGAGGTCTCTG-3’ |
DNMT3b-REVERSE | 5’-TGGTACATGGCTTTTCGATAGGA-3’ |
MYC-FORWARD | 5’-AGCGACTCTGAGGAGGAAC-3’ |
MYC-REVERSE | 5’-TGTGAGGAGGTTTGCTGTG-3’ |
HPRT-FORWARD | 5’-AGCCAGACTTTGTTGGATTTG-3’ |
HPRT-REVERSE | 5’-TTTACTGGCGATGTCAATAAG-3’ |