Fig. 4

Differentially expressed miRNAs beetween MYC translocation-positive and negative Burkitt lymphomas a Volcano plot on T-test for different miRNA expression between MYC translocation-positive and MYC translocation-negative. Volcano plot representing filtering threshold for one-tailed T-test for differential expression analysis between MYC translocation-positive and MYC translocation-negative. The plot shows the difference between the means of MYC translocation-positive and MYC translocation-negative for each miRNA plotted against the negative log10 p-value associated with the T-test. Black horizontal shows the threshold for p-value = 0,05 and red vertical lines are used for filtering miRNAs on fold change value of 1 and −1. All of the 4 points of the plot highlighted in red represent differentially expressed miRNAs that pass the filtering thresholds on p-value and fold change. b Hierarchical clustering on 4 differentially expressed miRNAs between MYC translocation-positive and MYC translocation-negative. Hierarchical cluster in samples and miRNAs for 4 differentially expressed miRNAs that passed filtering thresholds. Each row represents a miRNA and each column represents a sample. Similar samples and miRNAs of the experiment are connected by a series of branches. The length of each branch represents the distance in terms of Pearson correlation of log2(Hy3/Hy5) between connected samples or miRNAs. The miRNA clustering tree is shown on the left. The color scale shown at the top illustrates the relative expression level of a miRNA across all samples: red represents an expression level above the mean, green represents expression lower than the mean. The samples are colour coded according to the groups; yellow are the MYC translocation-positive (BL1-10), blue are the MYC translocation-negative. c Validation of miRNA profiling was assessed by RT-qPCR, which confirmed differential expression of these miRNAs in the two groups, being hsa-miR-29a and hsa-miR-29b down-regulated and hsa-miR-513a-5p, and hsa-miR-628-3p up-regulated in MYC translocation-negative BL cases