Fig. 2
From: Regulation of IL-8 gene expression in gliomas by microRNA miR-93
Expression of VEGF, IL-8 and miR-93 in Low-Grade Gliomas (LGGs) and High-Grade Gliomas (HGGs). VEGF mRNA (a) and IL-8 mRNA (b) levels relative to GAPDH were measured by RT-qPCR with TaqMan probes on RNAs isolated from FFPE sections of 6 LGG and 10 HGG and normalized to healthy brain reference RNA. Fold changes (FC) of expression over healthy brain reference RNA are reported. In the same LGGs and HGGs miR-93 was quantified (c) and normalized to healthy brain reference RNA. For panels a–c: dashed line: mean; solid line: median; grey box includes values from 5th to 95th centiles, vertical lines range from min to max values, excluding outliers which are represented by single dots. The data obtained in each glioma specimen are reported in the right side of the panels. d Relationship between VEGF mRNA and IL-8 mRNA in the same LGG (filled circles) and HGG (open circles) samples analyzed and reported in a–c. Regression straight line showing direct correlation was drawn by the least square method Sigmaplot. Inset reports the same graph expanded