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Fig. 4 | BMC Cancer

Fig. 4

From: Essential role of miR-200c in regulating self-renewal of breast cancer stem cells and their counterparts of mammary epithelium

Fig. 4

Verification of PDCD10 as a target of miR-200c. a. The binding sites of PDCD10 and TCF2-to miR-200c, and mFOLD analysis of free energy. b. Histogram of dual luciferase assay (a, compared with test group, P < 0.05). c. Experimental data of dual luciferase assay (a, compared with control groups, P < 0.05). d. In western blot assay, compared with empty vector (EV), Lenti-PDCD10 dramatically increases PDCD10 expression in BCSCs, and compared with miRNA control (miR-con), miR-200c agomir (miR-ag) dramatically decreases PDCD10 expression in BCSCs and MaSCs. e. In the 3-D matrigel culture, miR-200c agomir decreases, and Lenti-PDCD10 increases mammospheres of BCSCs compared with miR-control (P < 0.01, n = 5). Lenti-PDCD10 rescues mammospheres inhibited by miR-200c agomir (P < 0.01, n = 5)

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