Skip to main content
Fig. 3 | BMC Cancer

Fig. 3

From: Essential role of miR-200c in regulating self-renewal of breast cancer stem cells and their counterparts of mammary epithelium

Fig. 3

miR-200c inhibits the self-renewal of BCSCs and MaSCs. a. In qRT-PCR assay, miR-200c agomir significantly upregulates miR-200c expression in both BCSCs and MaSCs (*, P < 0.01); miR-200c antagomir significantly downregulates miR-200c expression in both BCSCs and MaSCs (**, P < 0.01). b. In MaSCs, miR-200c agomir significantly decreases the colonies (P < 0.01, n = 5) while miR-200c antagomir significantly increases the colonies (P < 0.01, n = 5). c. In BCSCs, miR-200c agomir significantly decreases colonies (P < 0.01, n = 5) while miR-200c antagomir significantly increases colonies (P < 0.01, n = 5). d. No tumor was observed in the test group (miR-200c agomir) in 2 months after inoculation of 10 K cells. In the miR-control group and parental BCSC group, average tumor volumes are 137.4 ± 13.7 mm3 and 124.1 ± 18.6 mm3, respectively. e. A limiting dilution assay for tumorigenesis in vivo and TIC Frequency calculation. f. Surface markers (ESA+CD44+CD24-/low) of BCSCs were detected on day 10 after transfecting miR-agomir or miR-control

Back to article page