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Fig. 4 | BMC Cancer

Fig. 4

From: Aberrant activation of NF-κB signaling in mammary epithelium leads to abnormal growth and ductal carcinoma in situ

Fig. 4

Many cells within aberrant ducts are epithelial, transgene-expressing, and have high levels of NF-κB activation. 6 week old virgin IKMV and control littermates were dox-treated (2 g/L) for 3 days prior to sacrifice. a Immunofluorescent staining of control and IKMV tissue reveals that IKMV ducts are filled with CK8/18 positive luminal epithelium. CK5 and SMA were used as markers of basal/myoepithelium. b Separate staining shows that the FLAG-tagged cIKKβ transgene is expressed by cells within the IKMV hyperplastic ducts (red, FLAG stain). In addition, high magnification images of ductal tissue from IKMV and control littermates confirmed that the transgene is localized appropriately within the cytoplasm of IKMV mammary epithelium and is driving concurrent nuclear localization of phospho-p65 (green)

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