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Fig. 2 | BMC Cancer

Fig. 2

From: Aberrant activation of NF-κB signaling in mammary epithelium leads to abnormal growth and ductal carcinoma in situ

Fig. 2

Expression of cIKKβ in transplanted mammary epithelium results in intraductal hyperplasia. Mammary tissue from 3–4 week old IKMV donors was transplanted into the cleared fat pad of the #4 mammary gland of 3 week old FVB recipients. Tissue from control littermates was transplanted into the contralateral #4 gland. After a 72 h recovery period, mice were placed on dox treatment (2 g/L), which was continuous until sacrifice at 3 or 4 weeks post-transplant. a Haematoxylin stained whole mounts of mammary fat pads after 3 weeks of growth in recipient mice reveal increased lateral branching of IKMV tissue. b Higher magnification highlights swollen end buds in IKMV. Hyperplasia of the IKMV ducts is evident in images of H&E stained tissue using c 10X and d 20X objectives. e Whole mounts of mammary tissue after 4 weeks of growth indicate that IKMV tissue continues to fill fat pad with hyperplastic tissue. The observed phenotype was quantitatively assessed through: f quantification of terminal end bud (TEB) size (n = 5 control, n = 6 IKMV glands, **p = 0.0071) g quantification of the number of lateral branch points per field (n = 4 control, n = 4 IKMV glands, *p = 0.0416)

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