Fig. 1

Transgenic mouse model targets expression of cIKKβ specifically to mammary epithelium. a Diagram shows crossing of two transgenic strains necessary to generate the double transgenic (*/*) IKMV mouse model with doxycycline inducible transgene expression. Littermates lacking either one or both transgenes (*/-, −/*, or −/−) were used throughout our studies as littermate controls. For characterization, IKMV and control littermates were treated with doxycycline (2 g/L) for 3 days and mammary tissue collected for the following assays: b RT-PCR of whole mammary homogenates confirms the FLAG-tagged transgene is dox-inducible. Upon dox-treatment, the transgene was expressed in the */* double transgenic IKMV animals, but absent in dox-treated, single transgenic control mice (−/*). Double transgenic */* IKMV mice that did not receive dox-treatment showed no detectable transgene expression. c TransAM ELISA assay using IKMV and control mammary nuclear homogenates shows that nuclear p65 in IKMV samples actively binds the NF-κB DNA consensus sequence (n = 4 control, n = 4 IKMV samples; **p = 0.0069)