Table 2 Primer sequences for amplifying SFRP genes
Gene | Forward | Reverse | bp | CpGs | Tm | |
|---|---|---|---|---|---|---|
SFRP1 | SFRP1_1 | aggaagagagTATGTGTGTTTGAGTGATGGATTTG | cagtaatacgactcactatagggagaaggctAAACAACACCTCTCCAAATAAAACC | 290 | 7 | 56 |
SFRP1_2 | aggaagagagAGTTTGGGAGGTTAAGGTAGGAGTA | cagtaatacgactcactatagggagaaggctAAAACCTAAATCATACTTACAAACCCAT | 300 | 12 | 56 | |
SFRP2 | SFRP2_1 | aggaagagagGGTTAGGTTTTTTTGTTTGTTGTTT | cagtaatacgactcactatagggagaaggctCACAACCAAAATTTTCTTAACCTTT | 235 | 9 | 56 |
SFRP2_2 | aggaagagagGGGGATGAATGAGTTAATTTTAGTT | cagtaatacgactcactatagggagaaggctAAAAATCTCACCAATCACACAAAAC | 296 | 9 | 56 | |
SFRP4 | SFRP4_1 | aggaagagagGTATGTGTGTTTGAGTGATGGATTT | cagtaatacgactcactatagggagaaggctTCCCAACTAACAAAAATTCAAAAAA | 314 | 6 | 56 |
SFRP4_2 | aggaagagagTTTTGAATTTTTGTTAGTTGGGAAA | cagtaatacgactcactatagggagaaggctAAAACCTAAATCATACTTACAAACCCA | 390 | 36 | 56 | |
SFRP5 | SFRP5_1 | aggaagagagGTATGTGTGTTTGAGTGATGGATTT | cagtaatacgactcactatagggagaaggctTCCCAACTAACAAAAATTCAAAAAA | 323 | 6 | 56 |
SFRP5_2 | aggaagagagTTTTGAATTTTTGTTAGTTGGGAAA | cagtaatacgactcactatagggagaaggctAAAACCTAAATCATACTTACAAACCCA | 329 | 6 | 56 | |