Fig. 1

Schematic diagram of the miR-200c/miR-141 locus and representative chromatograms of the PCR products. a Graphical depiction of the miR-200c/miR-141 genomic locus showing individual CpG sites as vertical lines and the pre-miR-200c and pre-miR-141 sequences as gray boxes. Arrows indicate the location of primers and delimitate the analyzed CGI that encompasses the region from −353 to −108 nucleotides, relative to the first nucleotide of the pre-miR-200c. The bottom bar is an enlargement of the analyzed CGI. b Representative sequencing chromatogram of the amplicon obtained from 25 % plasmid standard by using untailed primers (200c-BSP-F and 200c-BSP-R). Six out of 14 CpG are reported and indicated by gray arrows. c Part of the sequencing chromatogram of the Tail1-200c-F/Tail2-200c-R amplicon showing the Tail2 region. The black arrows indicate the C and the white arrows the T whose peak heights were used to determine the NF