Fig. 3

Expression of CRN5 increases cell migration velocity. a Lentivirus-mediated expression of a CRN5-specific shRNA caused an approximately 40 % reduction of both the CRN5 mRNA and protein levels in CT26 mouse colon carcinoma cells. A representative immunoblot and agarose gel as well as corresponding densitometry values are shown. CT26 control cells expressed a non-target (nt) shRNA. b Lentivirus-mediated GFP-CRN5 over-expression in U373 human glioblastoma cells led to a 3.8-fold increase of the total CRN5 protein level as compared to control cells expressing only GFP. A representative immunoblot as well as densitometry values are shown. c CT26 cells expressing the non-target or CRN5-specific shRNA (knock-down, kd) and (d) U373 cells over-expressing GFP or GFP-CRN5 were used for analysis of cell migration employing in vitro wound healing assays. Plot graphs show two-dimensional migration patterns of individual cells. Column charts indicate mean values and standard deviations of migration velocity and directionality (CT26 cell lines, n = 96 cells each; U373 cell lines, n = 150 cells each) as well as wound closure (CT26 cell lines, n = 6 gaps each; U373 cell lines, n = 10 gaps each)