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Fig. 4 | BMC Cancer

Fig. 4

From: Size is an essential parameter in governing the UVB-protective efficacy of silver nanoparticles in human keratinocytes

Fig. 4

Treatment with smaller size AgNPs modulates the expression of proteins related to survival of human keratinocytes. a HaCaT cells (1×106/plate) were seeded in UV transparent glass plates for 70 % confluence. Thereafter, cells were pretreated with AgNPs for 3 h prior UVB-exposure (40 mJ/cm2). After 24 h post UVB-exposure, total protein was isolated and subjected to immunoblot analysis for cell survival related proteins namely Bcl-xl, Bcl-2 and Bax followed by densitometry of immunoreactive bands. Normalized densitometric values are indicated at the top of the bands. β-actin was used as a loading control. b Bar diagram represents the Bax/Bcl-2 and Bax/Bcl-xL ratio in different treatment groups

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