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Fig. 8 | BMC Cancer

Fig. 8

From: The Deleted in Liver Cancer 1 (Dlc1) tumor suppressor is haploinsufficient for mammary gland development and epithelial cell polarity

Fig. 8

Knockdown of Dlc1 in primary mammary epithelial cells show defects in acinar lumen formation. ab Merged images showing green fluorescence protein expressed from the shRNA lentiviral vectors and DAPI (blue) in 3D Matrigel cultures. WT mammary epithelial cells infected with lentivirus expressing scramble control (a) or Dlc1 shRNA (b). Scale bar 100 μm. c Bar graph showing the percentage of solid acinar structures after lentivirus infection (p < 0.0001). d Quantitative RT-PCR analysis of Dlc1 mRNA levels following infection with Dlc1 targeting and scrambled control lentiviruses. Transcript levels were normalized to GAPDH (p = 0.0024). e Immunoblot analysis of Dlc1 protein levels in WT mammary epithelial cells infected with Dlc1 targeting and scrambled control lentiviruses. Four independent experiments with n = 3 animals in each group were used in this study

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