Fig. 4
Dlc1gt/+ mice show increased stromal collagen deposition in mammary glands. a–d Histological sections stained with H&E showed a thickened stromal layer in both the alveolar and ductal structures in the mammary glands from 10-week-old Dlc1gt/+ (b & d) compared with WT mice (a & c). These figures are representative of a total of 4 different glands (N = 4) from 4 different mice studied for each group. e–h Representative Masson’s trichrome staining (blue) of mammary glands from 10 week old virgin WT (e & g) and Dlc1gt/+ mice (f & h). The red arrows indicate areas of collagen deposition into the surrounding stroma. The fourth inguinal mammary glands from 3 different mice from each genotype were studied. Scale bar 100 μm