Fig. 1

Increased mammary terminal end buds/branch points in Dlc1^gt/+ mice. a–d Representative images of fourth inguinal mammary gland whole mounts of 10 week old virgin wild type mice (a & c) and Dlc1^gt/+ gene-trapped mice (b & d) on C57BL/6 background. Red arrowhead indicates terminal end buds (TEBs). Scale bar, 2 mm. e Bar graph showing that the total number of TEBs in 10 week old Dlc1^gt/+ compared to the wild type mammary glands. Data represents the mean ± SD of 5 different glands (N = 5) from 5 different mice for each group. f Quantification of the number of TEBs in the distal and proximal regions relative to the central lymph node. Data represents the mean ± SD. A total number of 5 different glands from 5 different mice were studied for each group by two-tailed Student’s t test, *p < 0.05. ns-not significant